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Introduction and Background
Bioanalytical
biotransducers comprise a fully contiguous and specifically
adhered (via covalent bond formation) thin film of
electroconductive polymer hydrogel fabricated on an inert
interdigitated microsensor electrode array device (IME).
These biotransducers serve as chemically-sensitive, solid-state
detection and monitoring devices possessing surface
functionality appropriate for the immobilization of your
bioactive indicator molecules.
Biotransducers
may be rendered biospecific by the covalent immobilization of
haptens, polypeptides, enzymes, antibodies, and DNA fragments.
With the associated Electroconductive Polymer Sensor
Interrogation System (EPSIS
240II CS), you
are provided with the computerized sensor interrogation and data
acquisition hardware for the simultaneous study of the biosensor
responses of these devices.
Only
the Full Differential (FD) device configuration and the 10
mincron line and space dimensions are used in the manufacture of
Bioanalytical Biotransducers. The FD confiiguration provides two
sensory areas on the single chip allowing for comparisons
between a biospecific element and its reference. The BB devices
are supplied in the form of a fully packaged (P) electrode with
leadwires attached and the device encapsulated using a
chemically resistant epoxy resin to seal the bonding pads into a
delrin electrode body.
Different surface
chemical functional groups [carboxylic acid (-COOH), primary
amine (-NH2), primary alcohol (-OH), or free
sulfonate (-SO3H) allows for the specific attachment
and immobilization of biologically active haptens, polypeptides,
enzymes, antibodies, enzyme-linked antibodies, DNA fragments, as
well as inorganic and other indicator molecules that may be of
interest in your research and development.
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Applications of BBs
Applications of BB in research and
product development include:
In
chemical and biosensor research and development: The high
sensitivity of these organic, polymeric, semiconducting films to
changes in redox state, along with the solid state construction
and small device size, make these devices ideally suited for
research and development of chemical and biosensor
applications.
As
chemoresistive sensor elements in multi-element arays:
Several differently responding, but singularly non-specific,
sensor elements may be assembled into multi-element arrays for
the recognition and monitoring of analytes of interest.
Biosensor line of products.
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Technical Specifications
Ø
Chip Substrate: Schott D263 Borosilicate Glass
Dielectric
Constant, Epsilon(r) at 1 MHz = 6.7
Dielectric
Loss Angle, tan delta, at 1 MHz = 61 x 10-4
Electrical
Resistivity (50 Hz) (250 C) = 1.6 x 108 ohm cm
Coefficient
of Linear Thermal Expansion, * 20-300 = 7.2 x 10^-6 K^-1
Refractive
Index at 20 C, ne ( Lambda = 546.1 nm) = 1.5249
Ø
Metallization: 100 Å Ti / 1000 Å Au or Pt
Digit
length, d, (microns) = 4,985
No.
of digit pairs per sensor, N = 50
Digit
Width, a, (microns) = 10
Interdigit
Space, a, (microns) = 10
Spatial
Periodicity, lambda, (microns) = 40
Zaretsky
Meander Length, M, (cm) = 24.93 cm
Center
Line or Serpentine Length (cm) = 49.55 cm
Cell
Constant (cm^-1) = 0.040 cm^-1
Ø
IME Chip Dimensions (l x w x t) for the Un-packaged Die
Monolithic,
M = 1.75 x 1.00 x 0.05
Combined
Differential, CD = 1.75 x 1.00 x 0.05 cm
Full
Differential, FD = 1.75 x 1.00 x 0.05 cm
Electrode
Body: Delrin
Encapsulant:
Epoxy header. Polyimide packaged chip.
Leadwires:
Color coded, 30AWG stranded copper, shielded, and PVC jacketed.
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Methods
Chemoresistance
responses of Bioanalytical Biotransducers may be obtained form a
wide assortment of interrogation hardware. These fall into three
categories.
Dedicated
Electroconductive Polymer Sensor Interrogation System (EPSIS).
DC
Resistance Measurements performed with:
Simple multimeters
set to resistance mode with operator recording of displayed
data.
Sophisticated multimeters with data logging capability
or computer interface (commonly RS232, IEEE488, or
USB).
Electrometers with computer interface (commonly RS232,
IEEE488, or USB).
Complex Impedance
response performed with:
Combined Function Generator and
Lock-in Amplifier.
Transfer Function Analyzers.
AC-Impedance
Analyzers.
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Application Notes
For further information,
request Application Notes or download
files:
IME1
- Interdigitated Microsensor Electrodes: Applications and
References.
IME2
- Conductimetric Urea Biosensor Formed From Interdigitated
Microsensor Electrodes
IME3
- Care and Cleaning Procedures
Electroconductive
Polymer Microsensor Electrode (EPME TM) is the
tradename for a family of non-specific sensor devices developed
by ABTECH.
Bioanalytical
Biotransducers is the tradename for a family of affinity and
activated sensors devices developed by ABTECH.
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Contact:
Ann M. Wilson, Manager,
Applications Development ABTECH
Scientific, Inc.
800 East Leigh Street, Suite G24, Richmond,
Virginia 23219
Telephone Number: +1-804-783-7829 Fax Number:
+1-804-783-7830
e-mail: sales@abtechsci.com
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